DATE2026.01.10 #Press Releases

“Mouse Circadian Proteome Atlas” Covering 32 Tissues Across the Whole Body

-Comprehensive Time-Resolved Analysis of ~19,000 Proteins Using the Next-Generation Mass Spectrometer Orbitrap Astral-

Summary

A research group led by Yuta Otobe, Research Scientist, and Hikaru Yoshitane, Project Leader (also Associate Professor at the Graduate School of Science, The University of Tokyo), from the Circadian Clock Project, Tokyo Metropolitan Institute of Medical Science, has constructed a comprehensive Mouse Circadian Proteome Atlas by using the next-generation mass spectrometry platform Orbitrap Astral.

In this study, the researchers performed comprehensive analyses of 24-hour changes in protein abundance and phosphorylation states across 32 tissues and brain regions in mice. As a result, they identified a total of 18,956 proteins from 584 samples, covering approximately 74% of all mouse proteins registered in UniProt. This achievement represents the most comprehensive proteome-wide “mouse clock map” to date.

All datasets obtained in this study are publicly available through the web-based resource Mouse Circadian Proteome Atlas (https://chronoproteinology.org/circadian_atlas).

This open-access platform enables users to explore when, where, and which proteins are active, providing a foundational resource for future research in circadian biology, physiology, and drug discovery.

The results of this study were published online in the international scientific journal Molecular Cell (Cell Press) on January 10, 2026 (Japan Standard Time).

Figure：Schematic overview of this study

Links

Tokyo Metropolitan Institute of Medical Science,
Tokyo Metropolitan Government, Bureau of Social Welfare and Public Health

Journals

Journal name	Molecular Cell
Title of paper	Mouse Circadian Proteome Atlas by next-generation deep proteome analysis